【文献标题】Short-chain aldehydes increase aluminum retention and sensitivity by enhancing cell wall polysaccharide contents and pectin demethylation in wheat seedlings
Pectin methylesterase activity was measured using an ELISA kit (Shanghai Hengyuan Biological Technology Co., Ltd). Approximately
0.1 g fresh weight of root tips (0–10 mm) was homogenized in 0.9 mL of PBS (50 mM, pH 7.0), and the extracts were centrifuged at 12,000g at 4 ℃ for 20 min. The crude enzyme extracts were used to determine the activities of pectin methylesterase by following the manufacturer’s instructions.
